REGULATION OF BACTERIAL BIOFILM DEVELOPMENT
RESEARCHERS INVOLVED
Fernando Govantes, Aroa López-Sánchez, Marta Pulido-Sánchez, María Zapata, Elisa Montero and Gonzalo Ariza
COLLABORATORS:
María Trinidad Gallegos (Estación Experimental del Zaidín-CSIC)
SUMMARY
In this line we investigate the mechanisms of physiological and genetic regulation of biofilm development in the plant growth-promoting bacterium Pseudomonas putida and the phytopathogenic bacterium Pseudomonas syringae. Our work has made a decisive contribution to the understanding of the mechanisms involved in biofilm formation and dispersal in response to nutritional stress in P. putida and has allowed us to identify some factors necessary for biofilm formation in P. syringae.
RELATED PUBLICATIONS
- Marta Pulido-Sánchez, , , HsbA represses stationary phase biofilm formation in Pseudomonas putida. BioRxiv
- Martínez-Rodríguez L, López-Sánchez A, García-Alcaide A, Govantes F and Gallegos MT. FleQ, FleN and c-di-GMP coordinately regulate cellulose production in Pseudomonas syringae pv. tomato DC3000. Front Mol Biosci. 2023 Mar 27;10:1155579. doi: 10.3389/fmolb.2023.1155579
- Govantes F. Serial dilution-based growth curves and growth curve synchronization for high-resolution time series of bacterial biofilm growth. Methods Mol Biol. 2018;1734:159-169. doi: 10.1007/978-1-4939-7604-1_13.
- Díaz-Salazar, Calero, P, Espinosa-Portero R, Jiménez-Fernández A, Wirebrand L, Velasco-Domínguez MG, López-Sánchez A , Shingler V and Govantes F. The stringent response promotes biofilm dispersal in Pseudomonas putida. Sci Rep. 2017 Dec 22;7(1):18055. doi: 10.1038/s41598-017-18518-0.
- Jiménez-Fernández A, López-Sánchez A, Jiménez-Díaz L, Navarrete B, Calero P, Platero AI and Govantes F. Complex interplay between FleQ, cyclic diguanylate and multiple σ factors coordinately regulates flagellar motility and biofilm development in Pseudomonas putida. PLoS One 2016 Sep;11(9):e0163142. doi:10.1371/journal.pone.0163142.
- Jiménez-Fernández A, López-Sánchez A, Calero P and Govantes F. The c-di-GMP phosphodiesterase BifA regulates biofilm development in Pseudomonas putida. Environ Microbiol Rep 2015 Feb;7(1):78-84
- López-Sánchez A, Jiménez-Fernández A, Calero P, Gallego LD and Govantes F. New methods for the isolation and characterization of biofilm-persistent mutants in Pseudomonas putida. Environ Microbiol Rep 2013 Oct;5(5):679-85 doi: 10.1111/1758-2229
